"Учебно-методический центр по гражданской
обороне, чрезвычайным ситуациям и пожарной
безопасности Приморского края"


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Also provided are methods of increasing levels (activity) of IDS in leukocytes of a subject, the methods comprising administering an intravenous solution as described herein (e.g., a system comprising three pharmaceutical compositions). Calculate the volume of HSA intravenous solution required to achieve a final concentration of 0.25% HSA, and (iv) Calculate the adjusted NS/PBS volume. In certain embodiments, the intravenous solution is delivered at a rate of 100 mL/hour. Any of the methods described herein may be delivered using an infusion pump, at any rate, for example, 10 to 200 mL/hour (or any value therebetween). I’m willing to take that step but I’m hesitant because we have a lot of open dialogue and he hasn’t seen a value in the past just due to the fact that we both feel like we can be open and honest without the need for another person in the room? Due to multiple current events in the business sector, employers are more vigilant regarding security measures, which has resulted in increased employment screening efforts. In another aspect, provided herein is an article of manufacture comprising one or more of the compositions described herein. In certain embodiments, the three components (ZFN1, ZFN2 and IDS donor) of the article of manufacture are added separately to an approximately 200 mL IV infusion bag, for example an IV infusion bag containing 0.25% HSA in NS or PBS

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> For example, streamate.Con – Nudecamsites.com, for a 50 kg subject to be treated at Cohort 1, 0.5e14 vg of ZFN1 (e.g. 47171 or 71557), 0.5e14 vg of ZFN2 (e.g. 47898 or 71729) and 4e14 SB-IDS are used. In certain embodiments, the donor is the donor designated SB-IDS AAV (e.g. Table 3, SEQ ID NO:17). In some embodiments, the components may be administered separately, or, preferably a composition comprising all components (paired ZFNs on the same or different vectors and IDS donor), for example a composition which comprises SB-47171 AAV (e.g., Table 1), SB-47898 AAV (e.g., Table 2) and SB-IDS AAV (e.g., Table 3). In some embodiments, the composition comprises SB-71557 AAV (e.g. Table 4, SEQ ID NO:30); SB-71728 (e.g. Table 5, SEQ ID NO:31); and SB-IDS AAV (e.g. Table 3, SEQ ID NO:17). One, two or three of the three pharmaceutical compositions may be individually formulated in phosphate buffered saline (PBS) containing CaCl2, MgCl2, NaCl, sucrose and a Poloxamer (e.g., Poloxamer P188) or in a Normal Saline (NS) formulation

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> Any concentration of the components can be used, including but not limited to the concentrations shown in Table 6. Further, any ratio of the three pharmaceutical compositions can be used, for example 1:1:8 (left ZFN:right ZFN:IDS donor). In some embodiments, the subject receives a total AAV dose, for example, of 1e13 vg/kg comprising 1e12 vg/kg of each ZFN AAV2/6 comprising either a left ZFN or a right ZFN, and 8e12 vg/kg of the hIDS donor AAV as disclosed herein. In other embodiments, the subject receives a total AAV dose, for example of 1e13 vg/kg comprising 1e12 vg/kg of each ZFN AAV2/6 comprising either a left ZFN or a right ZFN, and 8e12 vg/kg of the hIDS donor AAV as disclosed herein. In some embodiments, the subject receives a total AAV dose, for example, of 5e14 vg/kg comprising 5e13 vg/kg of each ZFN AAV2/6 comprising, for example, either a left ZFN or a right ZFN, and 4e14 vg/kg of the hIDS donor AAV. In some embodiments, ZFN expression is controlled by a liver-specific enhancer and promoter, comprised of the human ApoE enhancer and human .alpha.1-anti-trypsin (hAAT) promoter (Miao C H et al

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> In some embodiments, provided herein is a method of stabilizing, delaying, reducing or preventing the onset of a subject being wheelchair dependent in a human subject having MPS II as compared to a subject that that has not been treated with the methods and compositions as disclosed herein, the method comprising administering to the subject an effective amount of hIDS transgene and zinc finger nucleases (ZFN) wherein the subject has a stabilized, delayed, reduced or prevented onset of being wheelchair dependent after treatment. In some embodiments, the stabilized, delayed, reduced or the prevention of the onset of being wheelchair dependent is measured for the subject after a total dose of 5e12 vg/kg of the compositions described herein, of 1e13 vg/kg, of 5e13 vg/kg of 1e14 vg/kg, of 5e14 vg/kg and/or 1e15 vg/kg. In some embodiments, the extended life expectancy is measured for the subject after receiving a total dose of between 5e12 vg/kg to 1e15 vg/kg (for example, between 5e12 vg/kg and 5e13 vg/kg, between 5e12 vg/kg and 1e14 vg/kg, between 5e12 vg/kg and 5e14 vg/kg and/or between 5e12 vg/kg and 1e15 vg/kg)

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> He said that he would like to get his life together. Refusal, children at the moment are no extended in space or time as inoffensive as we like them to be, and in spite of the several carefulness person catch to keep from happening their teenager, from sexy web cam chat accommodation and bash energetic sex, it merely isn’t sufficient. But before workers at those companies took any big stands, Google employees were the poster children for protest in tech. It took us 30 years to get here – this band started in 1990 – and it’ll probably be another 30 years before we actually get out there and tour. We are both into running and started to hanging out together and going on runs together. Ashley Too. Also voiced by Cyrus, the literal teen idol doles out helpful advice to Rachel, a friendless, motherless teenager, and her punk sister Jack. It is not rational make enemies out of your allies as he is doing with his trade policies. Disclosed herein are methods and compositions for treating and/or preventing Hunter (MPS II) syndrome in a human subject comprising insertion of a suitable transgene sequence in a target cell

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